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Strategic Objective (4 B's) Best People & Practice Environment
Best Patient Experience
Best Quality
Best Financial Stewardship
Methodology Evidence Based Practice
Quality Improvement
Research
ANCC Magnet Model Component Transformational Leadership
Structural Empowerment
Exemplary Professional Practice
New Knowledge, Innovation, & Improvements
Empirical Quality Results
Project Type Unit Based Practice Council Initiative
Career Ladder Project
EBP Fellowship
Academic Activity
Other
Nurse Staffing and Safety of Patients Nurses are a key feature in the health care system, and they have a large impact on patient care. Their roles consist of direct patient care, assisting with activities of daily living (ADLs), medication administration, advocating for their patients, and many other tasks. The purpose of this evidence-based project was to determine if adequate nurse staffing produced better patient outcomes. To answer this question, information was gathered from research databases such as CINHAL, EBSCOhost, and PubMed. Keywords that were searched included, nurse staffing, patient outcomes, mortality rates, and quality of care. The results showed that when nurse staffing was inadequate there were increased rates of mortality, readmissions, increased costs, and poor work environments. It was also found that when nurse staffing was increased there was a direct improvement in the quality of care, workplace morale, patient experiences, and more cost-effective units. This led to the conclusion that when adequate nurse staffing is a p
Water Qualities in Selected Areas of Okmulgee County Introduction: Water is life. Considering the critical reliance that the earth has on water, the water qualities influence the human population, plants, and animals. Water quality is defined as being within the standard of federal and state regulations. One major source for water and nutrients is Deep Fork Refuge which provides shelter and food to over three hundred species of animals. Historically, Deep Fork has been known to be a site for human pollution. The Deep Fork Refuge pollutants could inflow to different Oklahoma water systems. Consequently animals may consume any pollution found at the refuge. Other sites such as Nichols Lake and Jim Hall Lake are water sources for a municipality and public recreational activities.
Methods: Areas which were studied in Okmulgee County included Deep Fork Refuge, Nichols Lake, and Jim Hall Lake. Chemical screenings were selected to determine the different toxins and the effects on the ecosystem within Deep Fork National Refuge. Other identified tests used were: Sali
Design of a Human-Centered Computing (HCC) Based Virtual Reality Simulator to Train First Responders Involved in the COVID-19 Pandemic Introduction: This research focuses on the design of a Virtual Reality (VR) simulator to help train nurses involved in testing and treatment of patients affected by the COVID-19 pandemic. This pandemic has placed an overwhelming strain on our Nation's ability to treat patients; the number of patients who need to be tested continues to rise. It is critical that our Nation have a larger pool of trained first responders. Currently, there is an urgent need to train such responders (nurses, physician assistants) to perform the testing and ventilator activities in a safe and efficient manner.
Microbiology in Fresh Water Aquarium Microorganisms surround us in our everyday lives. Some are even involved in our hobbies. An aquarium provides a passably controlled environment in which microorganisms can thrive and be isolated. The analysis was conducted to learn what types of microorganisms were needed to ensure a fish tank was a healthy and successful environment for its inhabitants. This experiment consisted of taking samples from various locations within a personal aquarium. The samples where incubated for a prescribed allotment of time. The samples where then placed onto a slide and analyzed under a compound microscope with various strengths of magnification. Many of the microorganisms found in the aquarium were the product of the carbon and nitrogen cycle taking place within the confines of the environment. At the conclusion of the paper, the experiment shed light on the microorganisms needed to preform those cycles efficiently and what would happen if an element of the cycle fell into disproportionate levels. Curiosity is what fueled
Identification and Characterization of an Important Metabolic Enzyme from Streptococcus sanguinis, Causative Agent of Subacute Infective Endocarditis In the United States, approximately 40,000 people will get diagnosed with subacute infective endocarditis (SIE) this year. A leading cause of this infection is a bacterium called Streptococcus sanguinis, which is present in the mouths of most of the human population. In certain populations, these bacteria can invade the heart valves and is the leading cause of SIE. Endocarditis is the inflammation of the heart and heart valves. Infective endocarditis, specifically, is the inflammation of the heart or heart valves due to bacteria. Bacteria can enter the bloodstream following everyday activities and can establish itself in the heart. Once bacteria invade the heart valves, it causes the heart valves to erode and commonly leads to heart failure. Without treatment, infective endocarditis is always fatal, and even with current treatment, patients have a mortality rate as high as 20%. Previous studies have focused on how the bacteria is able to attach to the surface of the heart, and the proteins that aid in this at
Comparative Study of National and State-wide COVID-19 Pandemic Trends Coronavirus (COVID-19) has been a current issue worldwide, and the prevalence of COVID-19 comparing to that of seasonal influenza (flu) has been an important topic in the public community. This study focuses on comparing national COVID-19 to flu in order to explore the epidemical features of COVID-19 for better understanding of this virus. The pandemic trend of Oklahoma has also been compared to national data to demonstrate the state’s standing in this pandemic event. Data were retrieved from the CDC, Worldometer, the COVID Tracking Project databases, and the Oklahoma Executive Order Report to look at the cases of cumulative, daily active, recovery, deaths, hospitalizations, ICU, and ventilator applications from 3/28/2020 to 10/31/2020. An unpaired, two-tailed, student t-test was used for statistical analysis. The results showed that there were two different COVID-19 peak phases which were phase one (P1) from 3/28/2020 to 6/15/2020 and phase two (P2) from 6/16/2020 to 10/31/2020. In general, COVID-19 showed h
Masked Meanings: Uncovering the Rhetorical and Symbolic Roles of the Face Covering in Anti-Mask Discourse At the heart of discourse surrounding the COVID-19 pandemic is an ongoing tension regarding the use of face masks, their efficacy in preventing the spread of the virus, and the extent to which authorities should enforce their use. The debate has led to the instilment of the mask as a symbolic image, weighty with polarized connotative meanings that depend on the ideological assumptions of different audiences. One audience views the mask as representative of oppression, blind obedience, and forced anonymity, while another understands mask-wearing as a gesture of precaution, courtesy, and community solidarity. The mask serves as the epicenter of ideological divisions that continually fight to attach to it their respective commonplaces and beliefs about the pandemic. The aim of this research is to examine how the mask functions rhetorically and how it mobilizes ideology and aids in the production of rhetorical arguments that shape public opinion about masks and the COVID-19 pandemic in general.
In my research,
Synthesis of Novel Solvent Molecules for use in Solvate Ionic Liquids Solvate ionic liquids (SILs) are a subgroup of ionic liquids in which an organic solvent, which contains atoms capable of donating one or more electron pairs, wraps around a solute cation molecule. In the context of petroleum research, SILs created from lithium salts may function as replacements for existing solvents in applications as diverse as heat transfer fluids, lubricants, or electrolyte solutions. Methyl capped ethylene glycols, or “glymes”, and crown ethers are commonly used for the molecular solvent portion of SILs. Replacing glymes with different molecular solvents which can coordinate with cations is a way to expand the array of properties allowed by SILs.
To study the formation and properties of new SILs and as a part of a larger project, this research focused on synthesizing novel solvent molecules by systematically replacing all or part of the oxygen atoms in glymes with tertiary amines. Six different solvents were targeted to be synthesized: 1,2-bis[2-(N,N-diethylamino)ethoxy]ethane (NOON),
Anti-Cancer Effects of Dandelion’s Extract on Cervical Cancer Cells Solvate ionic liquids (SILs) are a subgroup of ionic liquids in which an organic solvent, which contains atoms capable of donating one or more electron pairs, wraps around a solute cation molecule. In the context of petroleum research, SILs created from lithium salts may function as replacements for existing solvents in applications as diverse as heat transfer fluids, lubricants, or electrolyte solutions. Methyl capped ethylene glycols, or “glymes”, and crown ethers are commonly used for the molecular solvent portion of SILs. Replacing glymes with different molecular solvents which can coordinate with cations is a way to expand the array of properties allowed by SILs.
To study the formation and properties of new SILs and as a part of a larger project, this research focused on synthesizing novel solvent molecules by systematically replacing all or part of the oxygen atoms in glymes with tertiary amines. Six different solvents were targeted to be synthesized: 1,2-bis[2-(N,N-diethylamino)ethoxy]ethane (NOON),
Investigating Photophysical Properties of Cyanine Dye with and without Graphene Oxide Nanoparticles in Reverse Micelles In chemistry and biology the role of confined water is an important field. Among the most widely studied of confined media is the environment of the reverse micelle. Reverse micelles (RMs) are nanometer sized water droplets in organic solvents stabilized by surfactants. The RMs may be an appropriate model system to study photophysics of probe molecules and biomolecule dynamics in cellular environments. Therefore, the understanding of photophysics of probe molecules such as Cyanine 5 (Cy5) dye in RM is pivotal as it can be used as dye labels for biomolecules to track and analyze their behaviors inside biological cells. Moreover, the understanding of the effect of Graphene Oxide (GO) nanoparticles on photophysics of dye molecules is crucial due to their uses in nanomedicine and cellular imaging.
Exploring the Relationship Between Spirituality and Attitudes Toward Members of the LGBTQ+ Community In chemistry and biology the role of confined water is an important field. Among the most widely studied of confined media is the environment of the reverse micelle. Reverse micelles (RMs) are nanometer sized water droplets in organic solvents stabilized by surfactants. The RMs may be an appropriate model system to study photophysics of probe molecules and biomolecule dynamics in cellular environments. Therefore, the understanding of photophysics of probe molecules such as Cyanine 5 (Cy5) dye in RM is pivotal as it can be used as dye labels for biomolecules to track and analyze their behaviors inside biological cells. Moreover, the understanding of the effect of Graphene Oxide (GO) nanoparticles on photophysics of dye molecules is crucial due to their uses in nanomedicine and cellular imaging.
The Role of WRKY Transcription Factors in Virus Host Interactions During Drought In chemistry and biology the role of confined water is an important field. Among the most widely studied of confined media is the environment of the reverse micelle. Reverse micelles (RMs) are nanometer sized water droplets in organic solvents stabilized by surfactants. The RMs may be an appropriate model system to study photophysics of probe molecules and biomolecule dynamics in cellular environments. Therefore, the understanding of photophysics of probe molecules such as Cyanine 5 (Cy5) dye in RM is pivotal as it can be used as dye labels for biomolecules to track and analyze their behaviors inside biological cells. Moreover, the understanding of the effect of Graphene Oxide (GO) nanoparticles on photophysics of dye molecules is crucial due to their uses in nanomedicine and cellular imaging.
Boron Doped Carbon: A Tunable Morphology Our group has successfully synthesized a new mesoscopic material via an Atmospheric Chemical Vapor Deposition (APCVD) technique in an attempt to dope boron on a form of Pseudo-Graphite known as GUITAR. By adding a boron precursor into the solution, we discovered new mesoscopic structures that have formed with varying tubular morphologies. We call these novel structures BOD (Boron Orthocarborane Doped) Carbon and intend to explore their growth characteristics and possible applications. Some of these applications include hydrogen storage and improved battery technologies.
Analysis of ESCO2 Degradation using PIP-FUCCI Recombinant Plasmids Many proteins interact with cohesin to ensure the accurate alignment and segregation of sister chromatids during cell division. One of these proteins, the Establishment of Sister Chromatid Cohesion N-Acetyltransferase 2 protein (ESCO2), acetylates the SMC3 subunit of cohesin, which is essential for cohesion between the sister chromatids.
Task-Related Systemic Artifacts in Functional Near-Infrared Spectroscopy Introduction: Functional Near-Infrared Spectroscopy (fNIRS) is a neuroimaging technique that emits near-infrared light into the scalp and measures the light absorbed in the brain. It is relatively low-cost, portable, and noninvasive compared to other neuroimaging methods, such as functional magnetic resonance imaging (fMRI). However, data processing methods to improve fNIRS signals are still in their infancy.
Measuring Self-Control Using Structural Equation Modelling Introduction: Research has shown that individuals with higher levels of self-control are less likely to engage in deviant or illegal behavior. In order to explore this relationship empirically, several different statistical scales have been developed in an attempt to measure self-control in different ways using survey data. In 2003, Marcus (2003) created his Retrospective Behavioral Self-Control Scale (RBS) and argued that it outperformed the Grasmick scale in several important ways. The sample for his analyses came from Germany, and we have reason to expect the cultural difference between Germany and the United States might lead to these scales performing differently across these different cultural environments.
Design, Characterization and Bioanalysis of Micro-grooved Flow Diverting Stents Flow diverting stents have become one of the most promising endovascular treatment of intracranial aneurysms. Flow diverting stent, also known as flow diverter (FD), regulates blood flow and hemodynamic parameters to dissolve aneurysm and remodel vascular network. However, recent studies show that FDs may fail due to migration, malposition and dislodgment, and can cause constant inflammation, late thrombosis, and embolism. In this research, we developed novel micro-grooved nitinol FDs and studied its efficacy by human umbilical vein endothelial cell (HUVEC) adhesion, proliferation and differentiation analysis. Novel FDs with 3 um, 6 um and 12 um deep micro-grooved patterns were designed and developed using 3D CAD modeling and laser machining.
Assessing Public Knowledge and Habits of Battery Charging Although mobile phones have become integral in the workplace and society, we hypothesized that the public lacks knowledge – or is even misinformed – about the proper use and charging of lithium-ion batteries. Proper care is important because it leads to unnecessarily rapid decay in battery health.
Design of a Human-Centered Computing (HCC) Based Virtual Reality Simulator to Train First Responders Involved in the COVID-19 Pandemic Introduction: This research focuses on the design of a Virtual Reality (VR) simulator to help train nurses involved in testing and treatment of patients affected by the COVID-19 pandemic. This pandemic has placed an overwhelming strain on our Nation's ability to treat patients; the number of patients who need to be tested continues to rise. It is critical that our Nation have a larger pool of trained first responders. Currently, there is an urgent need to train such responders (nurses, physician assistants) to perform the testing and ventilator activities in a safe and efficient manner.
Subnaphthalocyanine Ensembles – Efficient Electron Acceptors in Organic Photovoltaics Reliable, renewable, and clean energy is growing in demand to keep up with our ever-growing society. With the consumption and depletion of the world’s sources of fossil fuels to meet the increasing demand that society has on energy resources, it is important to think of ways to obtain reliable energy without the consumption of the already limited supply of fossil fuels. One of the most important pieces of technology and innovation is the development of the photovoltaic cell or the solar cell. Photovoltaic cells use the photons emitted from the sun to interact with semiconductors to generate clean useful electricity. With the high demand of cheaper and cleaner sources of energy, there has been a major breakthrough in organic photovoltaics leading to the discovery of myriads of electron donor-acceptor conjugates for harvesting solar energy. When compared with subpththalocyanines (SubPc), the subnaphthalocyanine (SubNPc) entities have a longer pi conjugation system producing a red shift in the absorption spectru
TOBI VIRTUAL 2020 11th Annual Orthobiologics Virtual Symposium research posters.
PI1013/2 pages
Intra-articular Fat Derived Stem Cell Injections Effect On Pain, Stiffness, And Function In Osteoarthritis Analyze the impact of fat derived mesenchymal (MSC) injections using a Lipogems technique on pain, stiffness, and function associated with osteoarthritis (OA). We also evaluated trends in pain medication usage, Kellgren-Lawrence (KL) scales, and correlation with leukocyte poor platelet rich plasma (lp-PRP) injections.
Design / Intervention / Participants: A prospective observational study using questionnaires and Western Ontario and McMaster Universities Arthritis Index (WOMAC) Scores at 1 month (mo) prior to Stem Cell injections (SCI), 1 mo post SCI, and the recent month. 52 patients (86 joint injections) were treated with fat derived SCI, with/without a 1-2 week post lp-PRP injection for musculoskeletal (msk) issues (93% knee OA). WOMAC Scores were analyzed using a Within-Subject Repeated Analysis of variance (ANOVA) with alpha at .05, and a Bonferroni correction was applied.
Setting: The clinic of a Primary Sports Medicine physician at a local orthopedic practice in Anderson, SC.
Level of Evidence: Level 2
Monocyte Rich Platelet Rich Plasma for Osteonecrosis of the Femoral Head Intra-articular Fat Derived Stem Cell Injections Effect On Pain, Stiffness, And Function In Osteoarthritis
William Gasque, MD; Christopher Clemow, MD; Joel E. Williams, MPH, PhD; Mark Cromer, BS
AnMed Health Family Medicine Residency; Prisma Health- Blue Ridge Orthopaedics - Anderson
Objective / Main Outcome Measures:
To analyze the impact of fat derived mesenchymal (MSC) injections using a Lipogems technique on pain, stiffness, and function associated with osteoarthritis (OA). We also evaluated trends in pain medication usage, Kellgren-Lawrence (KL) scales, and correlation with leukocyte poor platelet rich plasma (lp-PRP) injections.
Design / Intervention / Participants:
A prospective observational study using questionnaires and Western Ontario and McMaster Universities Arthritis Index (WOMAC) Scores at 1 month (mo) prior to Stem Cell injections (SCI), 1 mo post SCI, and the recent month. 52 patients (86 joint injections) were treated with fat derived SCI, with/without a 1-2 week post lp-PRP injection for
Hypersensitivity Reaction After Intra-Articular Knee PRP Injection Adverse reactions to PRP injections are extremely rare. In the literature, there are only two documented case reports relating a hypersensitivity reaction to PRP injection. Here, we present a 63-year-old female who suffered an adverse reaction after undergoing an intra-articular PRP knee injection. An outside physician diagnosed her with serum sickness triggered by PRP injection based on her clinical symptoms. Documentation of adverse reactions and potential causes is necessary in order to obtain a more comprehensive risk profile of PRP.
Minimally Invasive Intraosseous Bioplasty for Treating Subchondral Cysts: A Case Report We were presented with 47-year old male with subchondral cyst (SC) in lateral condyle of femur with a history of chronic pain and no previous trauma. An Intraosseous Bioplasty (IOBP) was performed. First, diagnostic arthroscopy was performed to ensure no breach of femur articular surface. Bone-marrow was then harvested from the femur and processed in the Arthrex Angel system. The Arthrex Angel System separates platelet rich plasma (PRP) with nucleated cells, platelet poor plasma, and red blood cells (RBC’s) into separate sterile compartments with customizable levels of each including white blood cells (WBC’s). The bone marrow aspirate (BMA) is connected in the “whole blood in” compartment and spun for a total of 17min. The BMA is passed through three sensors to separate the blood components using the cell-specific wavelengths. The concentrated PRP that was obtained was mixed with demineralized bone matrix (DBM). The PRP concentration had roughly 105 x 10^3 platelets, greater than 1% WBC’s, less than 1% RBC’s
Multivalency enhances the specificity of Fc-cytokine fusions Interleukin (IL)-2 has potential as a therapy in cancer and autoimmunity but is limited in
effectiveness by its specificity toward desired immune populations. IL-2 muteins may have
improved cell type selectivity through altered receptor-ligand binding kinetics. Here, we analyze
the response of immune cells to a panel of IL-2 muteins in monomeric and dimeric Fc fusions
using tensor factorization. We find that dimeric muteins have considerably altered selectivity
profiles. We then dissect the mechanism of altered specificity in dimeric ligands using a
multivalent binding model and show that the enhanced selectivity by dimeric muteins arises due to multivalent ligands’ avidity for cells based on the abundance of their target receptors. Finally, we utilize this model to inform the design of tetravalent IL-2 muteins with potentially enhanced selectivity for regulatory T cells beyond what is achievable using mono- or bivalent variants. In total, we show that multivalent cytokines have unique selectivity profi
Investigating the Effect of ECM Stiffness in a 3D Biomimetic Liver Metastatic Niche Model for Pancreatic Cancer Pancreatic ductal adenocarcinoma (PDAC) is the 3rd leading cause of cancer death in US with a 5-year survival rate of only 11%. Early diagnosis is very difficult and thus 53% of patients are diagnosed after metastasis has already occurred, with liver being the most frequently affected site. Both primary tumors and metastases have highly fibrotic stroma, causing extracellular matrix (ECM) remodeling which leads to increased stiffness. Thus, models designed to recapitulate the stiff liver metastatic niche (LMN) are needed to develop therapies to eradicate metastatic lesions. To address this problem, we aim to design a biomimetic model that specifically recapitulates the LMN using a collagen rich ECM that mimics the metastatic site using LMN-derived fibroblasts, paired primary PDAC organoids, and matching LMN organoids derived from a mouse model of PDAC. CAFs make up a large portion of the tumor bulk in PDAC and are known to travel to the metastatic site. That is why we first investigated the role of primary sit
A multivalent vaccine to elicit potent humoral immune responses to Epstein-Barr virus infection and its associated cancers Primary infection with Epstein-Barr virus (EBV) is associated with acute infectious mononucleosis, while persistent infection is associated with chronic diseases such as autoimmunity and various types of cancers. Indeed, ~2% of all new cancer cases occurring annually worldwide are EBV-associated. Currently, there is no licensed EBV prophylactic vaccine. Selection of appropriate viral protein subunits is critical for an effective vaccine. Although the major EBV surface glycoprotein gp350/220 (gp350) has been proposed as a potentially important prophylactic vaccine target, attempts to develop a potent vaccine based on this target alone have shown limited success. In our laboratory, we have adopted the Modified Vaccinia Ankara (MVA) viral vector as a delivery platform for five EBV glycoproteins (gp350, gB, gp42, gL and gH) involved in virus entry and infection of diverse cell types permissive to EBV. We have constructed a recombinant MVA vector incorporating these five glycoproteins, and we show evidence that th
An Extracellular Vesicle Surface Protein Assay for Early Detection of Hepatocellular Carcinoma Background:
The sensitivity of current surveillance methods for detection of early-stage hepatocellular carcinoma (HCC) is suboptimal. Extracellular vesicles (EVs) are nanoparticles containing biomolecules such as DNA, RNA, and proteins. Given their presence in circulation during tumorigenesis, profiling tumor-derived EVs is regarded as a promising liquid biopsy strategy for early cancer detection. In this study, we aim to develop an HCC EV-based surface protein assay for detecting early-stage HCC.
Methods:
The expression of four potential HCC-associated surface protein markers, EpCAM, ASGPR1, CD147, and GPC3, was first evaluated using a 708-case HCC tissue microarray and EVs derived from HepG2 and Hep3B cells. An HCC EV Surface Protein Assay, composed of covalent chemistry-mediated HCC EV purification and real-time immuno-PCR analysis, was then developed and optimized for quantifying subpopulations of HCC EVs by adopting the four surface protein markers. Utilizing logistic regression, an HCC EV ECG was est
Preclinical mouse model of trypsin-dependent pancreatitis Background: Premature activation of trypsin in the pancreas is an early event in the development and progression of pancreatitis. Based on previous findings, this central step could be a potential pharmacological target to prevent the disease pathogenesis.
Human cationic trypsinogen (PRSS1) mutations accelerate trypsinogen autoactivation and cause hereditary chronic pancreatitis, a devastating disease with several complications. Therapeutic drug testing requires appropriate preclinical models, which recapitulate the trypsin-dependent human disease. Available mouse models of pancreatitis in the mouse cationic trypsinogen do not adequately model the human situation.
Our aim was to generate an improved preclinical mouse model, which develops trypsin-dependent spontaneous pancreatitis on a time scale that is more amenable for drug testing and detailed outcome analysis.
Conclusions: 1. The increased trypsinogen autoactivation can cause various manifestations of pancreatitis, and the rate of autoactivation is the k
A Role for SLC25A46 in Mediating Mitochondrial Dynamics Mediation of mitochondrial dynamics is essential to the health of an organism and its cells. If this process is disrupted, several diseases can result, including Pontocerebellar Hypoplasia (PCH), Charcot-Marie-Tooth Disease Type 2 (CMT2), and Optic Atrophy. Maintenance of a healthy mitochondrial network relies on a delicate balance between mitochondrial fission and fusion. The two processes must be coordinated across the inner and outer mitochondrial membranes, but how this occurs is not very well understood. Recently, we have identified a protein, SLC25A46, that may be responsible for coordinating the two processes. The protein is a member of the Solute Carrier Family but is localized to the outer mitochondrial membrane unlike the other solute carrier proteins, which are localized to the inner mitochondrial membrane. The protein has been shown to interact with several proteins including fusion regulators Mitofusin 1+2 (MFN1+2) on the outer membrane and Dynamin-like 120kDa Protein (OPA1) on the inner membrane
Development of off-the-shelf hematopoietic stem cell-engineered invariant natural killer T cells for COVID-19 therapeutic intervention New COVID‐19 treatments are desperately needed as case numbers continue to rise and emergent strains threaten vaccine efficacy. Cell therapy has revolutionized cancer treatment and holds much promise in combatting infectious disease, including COVID‐19. Invariant natural killer T (iNKT) cells are a rare subset of T cells with potent antiviral and immunoregulatory functions and an excellent safety profile. Current iNKT cell strategies are hindered by the extremely low presence of iNKT cells, and we have developed a platform to overcome this critical limitation. We produced allogeneic HSC‐engineered iNKT (AlloHSC‐iNKT) cells through TCR engineering of human cord blood CD34+ hematopoietic stem cells (HSCs) and differentiation of these HSCs into iNKT cells in an Ex Vivo HSC‐Derived iNKT Cell Culture. We reliably generated AlloHSC‐iNKT cells at high‐yield and of high‐purity; these resulting cells closely resem‐ bled endogenous human iNKT cells in phenotypes and functionalities. In cell culture, AlloHSC‐iNKT cells
V-SYNTHES 2.0 – A New Approach for the Screening Giga-Scale Space in Computer-aided Drug Design and Its Usefulness in Finding Novel Therapeutics Against Acute Respiratory Distress Syndrome Caused by COVID-19 V-SYNTHES 2.0 – A New Approach for the Screening Giga-Scale Space in Computer-aided Drug Design and Its Usefulness in Finding Novel Therapeutics Against Acute Respiratory Distress Syndrome Caused by COVID-19
Antonina L. Nazarova (a) (presenter), Arman A. Sadybekov (a), Anastasiia V. Sadybekov (a), Andrew J. Shepherd (b), Vsevolod Katritch (a)
(a)Department of Quantitative and Computational Biology, Michelson Center for Convergent Bioscience, University of Southern California, Los Angeles, CA 90089-3502, USA
(b)Department of Symptom Research, Division of Internal Medicine, MD Anderson Cancer Center, The University of Texas, TX 77030, USA
In recent years the chemical space of REadily AvailabLe (“REAL”) drug-like compounds has increased to 1015 compounds and continues to grow rapidly. However, the compound libraries used in high-throughput and virtual ligand screenings remain limited to 1-10 Mln compounds. The newly developed virtual synthone hierarchical enumeration screening (V-SYNTHES) approach in the Katri
Amyloid fibrils in disease FTLD-TDP are composed of TMEM106B, rather than TDP-43 Frontotemporal lobar degeneration (FTLD) is the third most common neurodegenerative condition, following only Alzheimer’s and Parkinson’s diseases. FTLD typically presents in 45-64-year-olds with behavioral changes or progressive decline of language skills. The subtype FTLD-TDP is characterized by certain clinical symptoms and pathological neuronal inclusions detected by TAR DNA-binding protein (TDP-43) immunoreactivity. Here, we extracted amyloid fibrils from brains of four patients, representing four out of five FTLD-TDP subclasses and determined their near-atomic resolution structures by cryogenic electron-microscopy (cryo-EM). Unexpectedly, all amyloid fibrils examined are composed of a 135-residue C-terminal fragment of transmembrane protein 106B (TMEM106B), a lysosomal membrane protein previously implicated as a genetic risk factor for FTLD-TDP. In addition to TMEM106B fibrils, abundant non-fibrillar aggregated TDP-43 is present, as revealed by immunogold labeling. Our observations confirm that FTLD-TDP
Identifying astrocyte and neuron plasma membrane proteins in vivo Astrocytes and neurons both contribute to diseases of the nervous system. Astrocytes are bushy cells that tile the entire central nervous system (CNS) and contribute, along with neurons, to the formation of complex networks with dense physical and functional connectivity between these cell types. Traditional methods to investigate plasma membrane proteomes have used either primary cell cultures or cells that have been acutely separated from tissue and have been purified based on cell surface markers for analysis. However, the resulting cells are no longer physiological and lose their cellular integrity, associated proteins, as well as most of their native connectivity. Consequently, the in vivo proteome of astrocytes and neurons in normal or dysfunctional states remains largely unknown. Therefore, to understand the role of astrocytes and their interactions with neurons at the protein level, we have designed a cell-specific method using the biotin ligase, BioID2, to tag proteomes in astrocyte and neuron plasma
Rejuvenation of neural stem cell function and cognition through single-cell pharmacotranscriptomics with ROOT Regenerative medicine offers the unrealized potential to reverse brain aging. However, endogenous neural stem cell (NSC) function declines with age of unclear mechanisms. Here, we developed a single cell computational drug screening technology to reinstate NSC activity, neurogenesis, and cognition in older mice through mimicking younger NSC programs. “Revealing Origins and Ontological Targets (ROOT)” first reconstructed single cell transcriptomes in cell-lineage pseudotime and identified transcriptomic signatures (TS) that define age-related NSC activity loss. ROOT then screened 1309 clinically relevant small molecules for their ability to recapitulate the TS gene networks mediating NSC activity. Three of the top 5 prioritized compounds reactivated NSCs from quiescence within middle-aged mice. Among them, SS further proved to sustain NSC proliferation, expand the NSC pool, increase neurogenesis, and improve cognitive function. We thus demonstrate the utility of a new single cell pharmacogenomics platform by r
Bioprinting of 3D Organoid Models Recapitulating Complex Tissue Architectures in Multi-well Plates Organoids, self-organized multicellular bodies in 3D extracellular matrices (ECMs), are gaining traction in basic biological research, drug discovery and precision medicine. Our lab has developed multi-well plate-based organoid models, “mini-“ or “maxi-rings”, with organoids derived from single cells embedded in gels deposited around the perimeter of tissue-culture wells, that simplify culture and facilitate the use of automated liquid handling platforms. However, manually seeding organoids, especially in 96-well plates, can be tedious. Therefore, automation of the organoid seeding process via bioprinting becomes an attractive option to minimize human labor and enhance consistency by eliminating variations resulting from operator skills and experience. Automated organoid seeding using bioprinting also opens possibilities for creating reproducible models of complex tissue architectures found in the body. In addition, our previous work has demonstrated how bioprinted organoids are not different from manually se
Small molecules stabilizing cell membrane for treatment of Duchenne muscular dystrophy Duchenne muscular dystrophy (DMD) is the most common lethal genetic disorder in children, characterized by progressive muscle wasting leading to severe muscle weakness, cardiac, and pulmonary failure. DMD is caused by loss of dystrophin, which normally forms a protein complex that connects the actin cytoskeleton, muscle membrane, and extracellular matrix. Sarcospan (SSPN) is a transmembrane protein that interacts with several major adhesion complexes. Overexpression of SSPN in the mouse model of DMD prevents the disease by restoring membrane stability. To identify pharmacological methods of increasing SSPN expression, we created a hSSPN promoter reporter assay and conducted an screened over 200,000 small molecules and rescreened the top 1,000 hits in two reporter cell lines. The hits were validated in both hSSPN EGFP and hSSPN-luciferase reporter cells. Hit selection was conducted on dystrophin-deficient mouse and human myotubes with assessments of 1) sarcospan gene expression and 2) sarcospan protein expres
Biodistribution of L-myc Immortalized Human Stem Cells from Olfactory Bulb to TBI sites in a Rat Model of Controlled Cortical Impact Injury Traumatic brain injury (TBI) often results in long-term neurological disabilities and affects ten million individuals worldwide each year (Hyder et al., 2007). In the United States, the incidence is 2.8 million per year (Taylor et al., 2017). Although various approaches have shown benefits in the laboratory, successful clinical translation has been essentially nonexistent. This dismal situation has motivated development of other alternative therapeutic strategies. Neural stem cells (NSCs) are attractive candidates for restoration of brain function after TBI as they inherently migrate to damaged sites, where they contribute neurotrophic factors to suppress inflammation, protect against further neuronal loss, promote recovery of existing damaged neurons, and possibly replace lost neurons and other cells (Gutova et al., 2013). However, a major challenge to successful NSC-based therapy is ensuring that sufficient numbers of cells reach damaged and non-functioning regions. We and others have explored intravenous
29th Annual Research Forum Every year, KU School of Medicine-Wichita holds a Research Forum for students, residents and faculty, as well as anyone interested in community health and medical research, to present and discuss research projects and facilitate research collaboration.
Expansion of community dental clinic to adults and children from immigrant, refugee, and asylee backgrounds. Dental caries is the most common condition present among recently arriving refugees, yet there remains a decline in their utilization of dental insurance. In 2014, Pew Research reported 240, 000 undocumented immigrants, of which 5.3% live in San Francisco, Oakland and Hayward areas.1 Resettling in a new country is an arduous task, and our project aims to alleviate this oral health disparity by working with the Bay Area Refugee Transitions to provide oral health education and referrals to the UCSF Free Community Dental Clinics (UCSF CDC) for comprehensive care.
ENHANCING THE HUMAN-ANIMAL CONNECTION IN SENIORS Senior citizens are often lonely and crave companionship. Studies have shown that the presence of a companion animal pet improves quality of life, emotional health and gives seniors something that enhances their lives.
Senior animals in shelters have a hard time getting adopted. They have longer stays in the shelter systems and are often the first to be euthanized.
Pairing the two helps the seniors find a companion and helps the animal find a loving home.
A Health & Social Justice Program for Young Women of Color in Oakland Oakland is a major hub for commercial exploitation due to the concentration of many foster care and group homes in the city that are prime areas for recruitment of young girls. Most recent estimates have identified over 200 minors being prostituted by 155 pimps.
More than half of exploited minors are Alameda County residents, identify as a racial minority, once lived in a group home, and report a history of physical or sexual assault.
Asian Health Services Youth Program (AHSYP) and Banteay Srei are community-based, culturally sensitive organizations that grew out of the need to serve young Southeast Asian women who are engaged in or at-risk of sexual exploitation.
We Are the Ones is a health and social justice art initiative that will provide support, counseling, healing arts, and leadership opportunities for the young women that AHSYP and Banteay Srei serve.
The Little Libraries: Addressing inequities in school readiness By age 3, low-income children hear 30 million fewer words than their more affluent peers. Reduced literacy at kindergarten is linked with continued poor developmental outcomes, lower high school graduation rates, and contribute to the intergenerational cycle of poverty.
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Bringing the Farm Back Home: Farm your Pharm 75% ($1.5 trillion) of our healthcare expenditures are coming from chronic diseases (CDC, 2019). With an ever-growing prevalence of over 1 in 2 Americans suffering from chronic disease (CDC, 2019), it is imminently clear that a change must be taken in the way we are currently combatting chronic disease. Utilizing the Structural Competency model, we can begin identifying upstream factors outside of the clinical vignette that have powerful effects on not only mortality and morbidity, but on the ability to receive and partake in behavioral changes for health.
Enhancing LGBTQ+ Affirming Clinical and Referrals Resources at the San Mateo County Pride Center In 2014, San Mateo County established California’s first county commission focused on the needs of their LGBTQ community.
The commission’s 2017 LGBTQ Community Wellness Survey identified culturally sensitive clinical health services and referrals as being especially needed, especially among “underserved and at-risk populations” within the community, including: youth, seniors, non-English speakers, and immigrants.
Intergenerational Volunteering Experiences for Individuals with Aphasia The Aphasia Access White Paper (Simmons-Mackie, 2017) reports that more than 2.5 million individuals in the United States are living with aphasia. Individuals with aphasia (IwA) often experience social isolation and decreased confidence in communication (Vickers, 2010; Babbit & Cherney, 2010). Despite having personal and social needs to re-engage in the community, aphasia may create barriers to being involved in meaningful social activities and relationships (Davidson et al., 2008). IwA are often viewed through the lens of disability, thus seen primarily as recipients of services which may limit a sense of personal agency and social equality. Opportunities to function in the role of
“expert” such as a volunteer are rarely available to them (Avent, 2009; Purves, 2013). Since social reintegration is a major life goal for many IwA, being involved in providing community service to others may help improve self-esteem, boost confidence, and increase a sense of personal value (Bhogal et al., 2003). Although there a
Implementation of STEM Experiences at a James Denman Middle School According to the National Action Council for Minorities in Engineering, ”Underrepresented minorities are particularly underrepresented in the fields of science, technology, engineering, and mathematics” (STEM).
MIND THE LINE Performance Improvement of Hand Hygiene Radiology Dept. identified that it could improve compliance with hand hygiene through education, availability of hand hygiene supplies, and defining patient care areas.
Patient Experience And Education Project (PEEP) The main goal of the project is to improve the RN Communication Scores to be adopted in a unit with new patient population, process flow and pace.
Discharge by Noon East 4’s UBPC attempted to increase discharges by noon by using four action plans. Our goal is to reach 17% discharged by noon (14.7% currently).
Change in Nursing Practice: CLABSI Prevention Central Line Associated Blood Stream Infection rates in the Surgical Intensive Care Unit continue to fluctuate despite hospital wide initiatives.
Implementation of an Anesthesia Handoff Checklist Tool Implementation of a standardized tool to use during anesthesia handoff to reduce omission of information, improve communication and improve the quality of handoff.
Biohazard Waste Reduction This project is aimed to reduce the amount of inappropriate biohazard bin/bag use. This will decrease the hospital's disposal cost and negative environmental impact.
Pressure Ulcer Project - East 5 HAPI Project E5 Rehab had its first Hospital Acquired Pressure Injury in 17 years. PG council conducted a fish tail to analyze possible causes and this lead us to educating our staff.
4-H:Leaders Start Here! 4H - organization serving youth to transform them into leaders. Recruitment and retention of volunteers is challenging. Recruitment portfolio and sequential plan created.
Pirkanmaa Ekothon 2020 The aim of the project is to pilot Pirkanmaa towards a controlled ecological transition instead of waiting for the crises that affect the sustainability of the planet to force us to give up the things that are necessary for our well-being.
KUMC - Faculty Research Day 2020 University of Kansas Medical Center Faculty Research Day 2020
KU School of Medicine, KU School of Medicine-Wichita, KU School of Nursing, and KU School of Health Professions
Klimathon 2020 The aim of the project is to pilot Pirkanmaa towards a controlled ecological transition instead of waiting for the crises that affect the sustainability of the planet to force us to give up the things that are necessary for our well-being.
Using a Learning Needs Assessment to Develop Infection Prevention Training for Certified Nursing Assistants in Skilled Nursing Facilities There are over 50,000 Certified Nursing Assistants (CNA) in California, making up over one third of skilled nursing facility (SNF) healthcare personnel (HCP). Despite makingup a large proportion of direct care HCP, CNA frequently lack understanding of core infection prevention and control (IPC) practices, including hand hygiene and appropriate personal protective equipment (PPE) use. The CDPH HAI Program developed new IPC training for SNF CNA, as part of the Centers for Disease Control and Prevention’s Project Firstline.
THE ELLIPTICITY OF p-INJECTIVE SUBGROUPS Let ρ ≥ −1 be arbitrary. We wish to extend the results of [30] to sub-conditionally
unique, sub-local, co-Brahmagupta factors. We show that there exists a left-almost stochastic
contra-almost differentiable equation. It would be interesting to apply the techniques of [30, 11] to locally right-Eratosthenes, prime functions. V. Perelman [30] improved upon the results of S. X. Jones by constructing left-almost everywhere anti-ordered, semi-complex, universally hyper-Euclid equations.
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