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Sanjeev Saxena(*), Salah Ahmed, Vinit Saxena, Roger Alsop
(*) ssaxena@Sepragen.com
Sepragen Corp.
ABSTRACT:
BACKGROUND Lactoferrin is a critical protein for infant nutrition and is derived from by extraction from either skim milk where it is present in very low concentrations or more recently from precision fermentation where higher titers can be engineered. The isolated and purified LF can then be reconstituted into formulae to mimic levels found in human breast milk.
Radial Flow Chromatography (RFC)
RFC has emerged as a scalable and cost-effective method for the large isolation of lactoferrin (LF) from large volumes of skim milk and now Pichia based Precision fermentation outputs as we search for sustainable alternatives to animal derived products.
Radial Flow Chromatography addresses traditional bottlenecks in protein purification by maximizing throughput and minimizing operational pressure drop. RFC employs a radial design chromatography column that enhances mass transfer efficiency and reduces processing time, making it well-suited for tonnage-scale production.
This study demonstrates the feasibility of Sepragen’s Superflo RFC Columns and Sepralac process for achieving high purity lactoferrin at industrial scales, highlighting significant cost savings compared to conventional methods. Optimized process conditions ensure high recovery yields and product quality, meeting stringent requirements for infant nutrition applications. The technology is applicable for extraction from both naturally bovine sourced lactoferrin as well the more promising and sustainable source such as Precision fermentation.
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